Chemical composition and in vitro ruminal degradation of diets containing Vachellia karro leaf meal and their effect on physiological responses of indigenous Xhosa lop-eared goats
- Authors: Mfisi, Khanyisa
- Date: 2021-09
- Subjects: Ruminants--Feeding and feeds , Animal nutrition
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20719 , vital:46460
- Description: The objective of this study was to determine the chemical composition and in vitro ruminal dry matter degradation (DMD) of Vachellia karro leaf meal-containing diets and their effect on feed intake and physiological responses of indigenous Xhosa lop-eared goats. Experimental diets containing 0 percent (Treatment 1), 15 percent (Treatment 2) and 30 percent (Treatment 3) Vachellia karroo leaf meal were mixed with grass hay and crushed maize. Samples of each diet were analysed for chemical composition and in vitro ruminal DM degradability. A total of 18 eight-month-old indigenous Xhosa lop-eared goats, initially weighing 20.81 ± 1.83 kg were randomly assigned to each diet in a Completely Randomized Design. The goats were confined in individual well-ventilated roofed pens and each dietary treatment had a total of 6 goats. The feed was offered at a rate of 3 percent of live weight twice a day, at 08:00 in the morning and at 16:00 in the afternoon. The experimental feeding trial lasted for 80 days, where 10 days was allowed for adaptation. Goat live weight, Average daily feed intake (ADFI), and Body condition scores (BCS) were recorded weekly FAMACHA scores and fecal eggs counts were also determined weekly. Blood samples were collected via vein puncture on the last day of the feeding trial for determination of glucose, total cholesterol, albumin and blood urea. Significantly higher (P < 0.05) crude protein (CP) and condensed tannins (CT) levels were observed in Vachellia karroo containing diets., while acid detergent fibre (ADF) and neutral detergent fibre (NDF) were significantly higher (P < 0.05) in the control diet. During early hours of incubation, DMD was similar (P > 0.05) across all dietary treatments. At 12 to 72 hours, the DMD was significantly higher (P < 0.05) in Vachellia karroo containing diets except at 48 hours. Correlation between DMD and NDF, ADF and fat was only visible at 24 hours. Fibre (ADF and NDF) were negatively related to DMD, while fat was positively related. A positive correlation was observed between DMD and DM, CP and ADFI. The body weight and BCS of goats was similar (P > 0.05) in all dietary treatments (P > 0.05). The ADFI was significantly higher (P < 0.05) in Vachellia karroo supplemented goats. Faecal egg count and FAMACHA scores were significantly higher (P< 0.05) in the control group compared to the Vachellia karroo supplemented treatments; while glucose levels were significantly higher in goats supplemented with Vachellia karroo compared to non-supplemented goats. Cholesterol levels were similar (P > 0.05) across all experimental groups. Goats consuming 30 percent of Vachellia karroo leaf meal had higher (P < 0.05) creatinine levels than the non-supplemented treatment. Urea levels were significantly higher (P < 0.05) in goats fed 30 percent of Vachellia karroo leaf meal than other treatments. It was concluded that the inclusion of Vachellia karroo leaves improves diet quality, diet degradability, average daily feed intake and physiological responses. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
- Authors: Mfisi, Khanyisa
- Date: 2021-09
- Subjects: Ruminants--Feeding and feeds , Animal nutrition
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/20719 , vital:46460
- Description: The objective of this study was to determine the chemical composition and in vitro ruminal dry matter degradation (DMD) of Vachellia karro leaf meal-containing diets and their effect on feed intake and physiological responses of indigenous Xhosa lop-eared goats. Experimental diets containing 0 percent (Treatment 1), 15 percent (Treatment 2) and 30 percent (Treatment 3) Vachellia karroo leaf meal were mixed with grass hay and crushed maize. Samples of each diet were analysed for chemical composition and in vitro ruminal DM degradability. A total of 18 eight-month-old indigenous Xhosa lop-eared goats, initially weighing 20.81 ± 1.83 kg were randomly assigned to each diet in a Completely Randomized Design. The goats were confined in individual well-ventilated roofed pens and each dietary treatment had a total of 6 goats. The feed was offered at a rate of 3 percent of live weight twice a day, at 08:00 in the morning and at 16:00 in the afternoon. The experimental feeding trial lasted for 80 days, where 10 days was allowed for adaptation. Goat live weight, Average daily feed intake (ADFI), and Body condition scores (BCS) were recorded weekly FAMACHA scores and fecal eggs counts were also determined weekly. Blood samples were collected via vein puncture on the last day of the feeding trial for determination of glucose, total cholesterol, albumin and blood urea. Significantly higher (P < 0.05) crude protein (CP) and condensed tannins (CT) levels were observed in Vachellia karroo containing diets., while acid detergent fibre (ADF) and neutral detergent fibre (NDF) were significantly higher (P < 0.05) in the control diet. During early hours of incubation, DMD was similar (P > 0.05) across all dietary treatments. At 12 to 72 hours, the DMD was significantly higher (P < 0.05) in Vachellia karroo containing diets except at 48 hours. Correlation between DMD and NDF, ADF and fat was only visible at 24 hours. Fibre (ADF and NDF) were negatively related to DMD, while fat was positively related. A positive correlation was observed between DMD and DM, CP and ADFI. The body weight and BCS of goats was similar (P > 0.05) in all dietary treatments (P > 0.05). The ADFI was significantly higher (P < 0.05) in Vachellia karroo supplemented goats. Faecal egg count and FAMACHA scores were significantly higher (P< 0.05) in the control group compared to the Vachellia karroo supplemented treatments; while glucose levels were significantly higher in goats supplemented with Vachellia karroo compared to non-supplemented goats. Cholesterol levels were similar (P > 0.05) across all experimental groups. Goats consuming 30 percent of Vachellia karroo leaf meal had higher (P < 0.05) creatinine levels than the non-supplemented treatment. Urea levels were significantly higher (P < 0.05) in goats fed 30 percent of Vachellia karroo leaf meal than other treatments. It was concluded that the inclusion of Vachellia karroo leaves improves diet quality, diet degradability, average daily feed intake and physiological responses. , Thesis (MSc) (Animal Science) -- University of Fort Hare, 2021
- Full Text:
- Date Issued: 2021-09
Evaluation of near infrared reflectance (NIR) spectroscopy to determine the nutrient composition of raw materials and compound ostrich feeds
- Swart, Etheresia, Lehmann-Maritz, Maryna
- Authors: Swart, Etheresia , Lehmann-Maritz, Maryna
- Date: 2017
- Subjects: Near infrared spectroscopy , Animal nutrition , Animal feeding
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13779 , vital:27307
- Description: The chemical analysis of feed samples can be time consuming and expensive. The use of near infrared reflectance (NIR) spectroscopy was evaluated in a range of studies as a rapid technique to predict the chemical constituents in feedstuffs and compound ostrich feeds. The prediction of accurate results by NIR spectroscopy relies heavily upon obtaining a calibration set which represents the variation in the main population, accurate laboratory analyses and the application of the best mathematical procedures. This research project was designed to meet five objectives: The first objective was to determine the feasibility of using near infrared reflectance (NIR) spectroscopy to predict dry matter, ash, crude protein, crude fibre, oil content, and fatty acids such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) in sunflower seed meal. The second objective was to develop calibration models to predict the dry matter, crude protein and oil content in milled canola seed, compared to whole canola seeds. The third objective was to investigate the feasibility of using NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre and oil content in milled lupin seeds, compared to whole lupin seeds. The fourth objective was to describe the development of near infrared reflectance (NIR) spectroscopy calibration equations for the prediction of chemical composition and amino acid content from different populations of alfalfa hay (Medicago sativa L.). The last objective was to determine the potential of NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre, ether extract, acid detergent fibre (ADF), neutral detergent fibre (NDF), calcium, phosphorus, in vitro organic matter digestibility (IVOMD) and amino acids such as lysine, methionine, threonine and arginine in compound ostrich feed samples. The results of this study indicate that NIR spectroscopy calibrations in sunflower seed meal are only applicable in sunflower breeding programmes for a fast screening as it was not suitable for prediction purposes. Screening of sunflower seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that is a great utility for users who need to analyse a large number of samples. Calibrations developed for crude protein and oil content in milled canola seeds proved to be better than calibrations for whole canola seeds. Although the results indicated that calibrations were better for milled canola seeds, it indicated values that were typical of equations suitable for screening purposes to select samples for more detailed chemical analysis. According to calibration statistics obtained for crude protein, crude fibre and oil content in whole lupin seeds, there is no need to grind the seeds to scan the meal as similarly accurate results were obtained by analysing whole seeds. Screening of whole lupin seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that may be of great utility for users who need to analyse a large number of samples with no sample preparation. The calibration and validation statistics obtained in the study to test the potential of NIR spectroscopy to predict the chemical composition and amino acid contents in alfalfa hay, showed the accuracy was too low for routine analysis, although NIR spectroscopy could be used as a screening tool. Further research needs to be done to improve the accuracy of the NIR spectroscopy analysis, including more samples from different cultivars and years. In the study to examine the possibility of using NIR spectroscopy to predict the chemical composition of compound ostrich feeds, the results indicated that NIR spectroscopy is a suitable tool for a rapid and reliable prediction of the crude protein, crude fibre, ether extract, IVOMD, ADF and NDF in compound ostrich feeds. Calibrations can be improved for amino acids if a larger sample pool is used to develop the calibrations. These studies indicated that NIR spectroscopy can be a rapid and successful tool for the prediction of the nutritive value up to certain amino acid contents of feedstuffs and compound ostrich feeds.
- Full Text:
- Date Issued: 2017
- Authors: Swart, Etheresia , Lehmann-Maritz, Maryna
- Date: 2017
- Subjects: Near infrared spectroscopy , Animal nutrition , Animal feeding
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13779 , vital:27307
- Description: The chemical analysis of feed samples can be time consuming and expensive. The use of near infrared reflectance (NIR) spectroscopy was evaluated in a range of studies as a rapid technique to predict the chemical constituents in feedstuffs and compound ostrich feeds. The prediction of accurate results by NIR spectroscopy relies heavily upon obtaining a calibration set which represents the variation in the main population, accurate laboratory analyses and the application of the best mathematical procedures. This research project was designed to meet five objectives: The first objective was to determine the feasibility of using near infrared reflectance (NIR) spectroscopy to predict dry matter, ash, crude protein, crude fibre, oil content, and fatty acids such as palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1) and linoleic acid (C18:2) in sunflower seed meal. The second objective was to develop calibration models to predict the dry matter, crude protein and oil content in milled canola seed, compared to whole canola seeds. The third objective was to investigate the feasibility of using NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre and oil content in milled lupin seeds, compared to whole lupin seeds. The fourth objective was to describe the development of near infrared reflectance (NIR) spectroscopy calibration equations for the prediction of chemical composition and amino acid content from different populations of alfalfa hay (Medicago sativa L.). The last objective was to determine the potential of NIR spectroscopy to predict the dry matter, ash, crude protein, crude fibre, ether extract, acid detergent fibre (ADF), neutral detergent fibre (NDF), calcium, phosphorus, in vitro organic matter digestibility (IVOMD) and amino acids such as lysine, methionine, threonine and arginine in compound ostrich feed samples. The results of this study indicate that NIR spectroscopy calibrations in sunflower seed meal are only applicable in sunflower breeding programmes for a fast screening as it was not suitable for prediction purposes. Screening of sunflower seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that is a great utility for users who need to analyse a large number of samples. Calibrations developed for crude protein and oil content in milled canola seeds proved to be better than calibrations for whole canola seeds. Although the results indicated that calibrations were better for milled canola seeds, it indicated values that were typical of equations suitable for screening purposes to select samples for more detailed chemical analysis. According to calibration statistics obtained for crude protein, crude fibre and oil content in whole lupin seeds, there is no need to grind the seeds to scan the meal as similarly accurate results were obtained by analysing whole seeds. Screening of whole lupin seeds by NIR spectroscopy represents a rapid, simple and cost effective alternative that may be of great utility for users who need to analyse a large number of samples with no sample preparation. The calibration and validation statistics obtained in the study to test the potential of NIR spectroscopy to predict the chemical composition and amino acid contents in alfalfa hay, showed the accuracy was too low for routine analysis, although NIR spectroscopy could be used as a screening tool. Further research needs to be done to improve the accuracy of the NIR spectroscopy analysis, including more samples from different cultivars and years. In the study to examine the possibility of using NIR spectroscopy to predict the chemical composition of compound ostrich feeds, the results indicated that NIR spectroscopy is a suitable tool for a rapid and reliable prediction of the crude protein, crude fibre, ether extract, IVOMD, ADF and NDF in compound ostrich feeds. Calibrations can be improved for amino acids if a larger sample pool is used to develop the calibrations. These studies indicated that NIR spectroscopy can be a rapid and successful tool for the prediction of the nutritive value up to certain amino acid contents of feedstuffs and compound ostrich feeds.
- Full Text:
- Date Issued: 2017
How phase feeding manipulation affects growth, performance, feed cost, carcass characteristics and the quality of meat from broilers
- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
- Full Text:
- Date Issued: 2017
- Authors: Sokanyile, Sanda
- Date: 2017
- Subjects: Animal nutrition , Feeds , Broilers (Chickens)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10353/5565 , vital:29334
- Description: The main objective of the study was to determine how phase feeding manipulation affects growth performance, feed cost, carcass characteristics and quality of meat from broiler chickens. A total of 180 day old un-sexed broiler chicks (Cobb 500) were separated into 3 dietary treatments of 60 birds per treatment and each treatment was replicated 3 times with 20 birds per replicate. The dietary treatments were as follows: T1 (starter 1-7 days; grower 8-21 days; finisher 22-35 days), T2 (starter 1-14 days; grower 15-21 days, finisher 22-35 days) and T3 (starter 1-14 days; grower 15-28 days; finisher 29-35 days). Feed intake and body weight gains were recorded weekly in kilograms. Average daily gain and feed conversion ratio were computed weekly. At day 35, the chickens were slaughtered and 12 representative breast muscles from each treatment were used for the determination of muscle pH (pH1, pH24 and pH48) and colour coordinates (Lightness – L*, redness – a* and yellowness – b*) in triplicate at 1 hour, 24 hours and 48 hours post-mortem.Feed intake (FI), feed conversion ratio (FCR), average daily gain (ADG) and average body weight at slaughter (ABW) were not significant (P>0.05) amongst the treatments. The most cost efficient feed (which accumulated the cheapest cost feed per kg were) T1 (R11.32) and T3 (R11.32) although there was a slight difference in the gross profit of these treatments T1 (R45.71) and T3 (R44.48). Carcass characteristics were the same (P > 0.05) across the treatments except for the wing, thigh, drumstick and the breast (P < 0.05). T2 had the highest wing weight (166.63±8.60), T1 (113.03±8.60) and the lowest in T3 (74.46±8.60). The thigh weight were greater (P<0.05) in T2 (185.69±4.34); T1 (185.54±4.34) compared to T3 (166.97±4.34) which was lower. Treatments 1 (204.17±6.57), T2 (197±6.57) had heavier (P<0.05) breast weights than T3 (186.06±6.57). Dietary treatments had no significant effect (P > 0.05) on pH of the breast meat. No differences were observed in colour during the 1 hour period after slaughter. At 24 hours after slaughter, the L* values were different (P < 0.05) in T1 and T2 (44.3 ± 0.37; 43.7 ± 0.37), respectively, T1 and T3 were the same. The a*-values were different (P < 0.05) at 48 hours after slaughter for T1 and T3 (4.5 ± 0.27; 3.4 ± 0.27), T2 was the same as T1. It was therefore, concluded that since manipulation of starter phase did not have adverse effects on growth performance, gross profit, slight statistical difference in meat quality attributes and carcass characteristics. Therefore, the manipulation of starter diet has the potential to be used in broiler production.
- Full Text:
- Date Issued: 2017
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17527 , http://hdl.handle.net/10353/d1009812
- Description: Animal Nutrition: AGA 321, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17527 , http://hdl.handle.net/10353/d1009812
- Description: Animal Nutrition: AGA 321, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
Principles of Animal Nutrition: AGA 221
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17519 , http://hdl.handle.net/10353/d1009784
- Description: Principles of Animal Nutrition: AGA 221, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2010-02
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17519 , http://hdl.handle.net/10353/d1009784
- Description: Principles of Animal Nutrition: AGA 221, supplementary examination February 2010.
- Full Text: false
- Date Issued: 2010-02
Feeding Practices: AGA 607
- Nkukwana, T, Chimonyo, M, van de Vyver, F
- Authors: Nkukwana, T , Chimonyo, M , van de Vyver, F
- Date: 2010-01
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17530 , http://hdl.handle.net/10353/d1009823
- Description: Feeding Practices: AGA 607, supplementary examination January 2010.
- Full Text: false
- Date Issued: 2010-01
- Authors: Nkukwana, T , Chimonyo, M , van de Vyver, F
- Date: 2010-01
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17530 , http://hdl.handle.net/10353/d1009823
- Description: Feeding Practices: AGA 607, supplementary examination January 2010.
- Full Text: false
- Date Issued: 2010-01
Animal Nutrition: AGA 321
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17553 , http://hdl.handle.net/10353/d1009881
- Description: Animal Nutrition: AGA 321 Degree examination November 2009
- Full Text: false
- Date Issued: 2009-11
- Authors: Nkukwana, T , Chimonyo, M
- Date: 2009-11
- Subjects: Animal nutrition
- Language: English
- Type: Examination paper
- Identifier: vital:17553 , http://hdl.handle.net/10353/d1009881
- Description: Animal Nutrition: AGA 321 Degree examination November 2009
- Full Text: false
- Date Issued: 2009-11
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